FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666418
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT7220.10834041097329304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25550.046.7509271
GTGGTAT5300.043.310451
TATCAAC28250.042.7212872
TCAACGC29700.040.3494034
TGGTATC5200.040.0439762
ATCAACG30000.039.9459083
GGTATCA11400.039.898241
CAACGCA30350.039.485255
AACGCAG32200.037.348666
ACGCAGA32450.037.058147
CGCAGAG32450.036.9299628
TATATAG601.5957716E-435.413042
GCAGAGT35100.034.1418049
GTATATA1106.4809283E-627.0508041
TATTAGG1509.808173E-825.497392
CAGAGTA32150.024.915810-11
GCTTATA1302.3656385E-522.8891411
TACGAGA1302.3711349E-522.882274
AGAGTAC31050.022.58222810-11
GACAGGT3400.022.5044521
Produced by FastQC (version 0.11.5)