FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666418
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17970.26965057966621553No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT401.9068267E-434.356157
GTGGTAT4650.032.538341
GGTATCA12000.032.0946351
TGGTATC4750.031.8389762
GTATCAA27000.030.566321
CCTATCG400.0078630427.50555840
TATCAAC29850.027.4512392
TGCCGAC3050.026.15282455
TCAACGC31700.025.7547194
ATCAACG32050.025.6469293
CAACGCA32450.025.2403875
ACGCAGA33400.024.5224727
AACGCAG33650.024.4219656
TACGGGT3500.024.35840815
CGCAGAG33800.024.150958
AGAGTAC33350.023.99134411
CAGAGTA33650.023.94087210
GCAGAGT35200.023.1192579
ATATACT852.5761461E-522.6380354
CGGGTCA1254.6136847E-822.00114317
Produced by FastQC (version 0.11.5)