FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691903
Sequences flagged as poor quality0
Sequence length61
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12450.17993851739333402No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3050.031.5492042
GTATCAA15250.030.309151
GTGGTAT3500.029.084931
TATCAAC17300.027.1750412
GGTATCA7400.026.3973881
TCAACGC18500.024.9556794
ATCAACG18750.024.919673
CAACGCA18650.024.7585435
AACGCAG19000.024.4488896
ACGCAGA19350.023.8646097
CGCAGAG20050.022.8976488
AGAGTAC19800.022.63957411
CAGAGTA20050.022.49281710
GCAGAGT21600.021.2606819
ATCGCAT804.4389055E-420.62562414
AGTACGG22750.019.82477613
TTAGACC700.00499403519.6292554
CTGCCGA5550.018.8307655
GAGTACG24300.018.56023212
CTCTAAT909.779726E-418.3418481
Produced by FastQC (version 0.11.5)