FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310548
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT3670.11817818823499104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1900.044.734552
GTATCAA7350.044.5215341
GTGGTAT2300.038.8023641
TATCAAC8650.037.8304332
ATCAACG8750.037.3980873
CAACGCA8950.036.5623745
TCAACGC9100.035.95974
GGTATCA3850.035.322871
AACGCAG9700.034.173516
CGCAGAG10000.032.7233248
ACGCAGA10300.032.182827
GTATGGA550.00444417230.9075091
TATCCTT600.006799747728.3318845
TACCGGT300.006804909628.33188438-39
GCAGAGT11750.027.849649
GTCCTAG850.001224903724.9987221
ACAGGTA1201.2728075E-524.79041
CAGTACT1051.4677638E-424.2844724
TTATCCT900.001708772823.6099034
ACGGGAT2350.023.50943616-17
Produced by FastQC (version 0.11.5)