FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310548
Sequences flagged as poor quality0
Sequence length61
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9050.2914203279364221No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAT150.005776297355.00805717
GGTATCA4050.035.3195081
AAGTCGC350.004105059431.4129267
GTATCAA8700.030.9865441
TATCAAC9500.028.0694892
ACACTTT400.00785880427.5040331
TAAATTA400.00785880427.5040335
GAGCGTT400.00788356227.4863117
ATCAACG9850.027.0633773
CAACGCA10200.026.4041635
CGGGGTA1057.205017E-926.19431517
TCAACGC10200.026.1347334
TGGTATC957.958624E-826.0438562
ACGGGGT1800.025.97602716
AGAGTAC10400.025.64866411
AACGCAG10700.025.1743776
GGATCGA1001.3014323E-724.75362419
ACGCAGA10900.024.7124617
CAGAGTA11100.024.52271710
CGCAGAG11000.024.4878048
Produced by FastQC (version 0.11.5)