FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237844
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT3020.12697398294680548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6100.051.5774081
GTGGTAT1350.044.0911981
TATCAAC7500.043.0743372
ATACTAC409.3059515E-442.4986343
GGTATCA2850.041.7706071
ATCAACG7650.041.6653253
CAACGCA7900.039.8088465
TCAACGC7900.039.8088464
ACGCAGA8400.038.4511457
TGGTATC1550.038.3939322
CGCAGAG8250.038.1199878
AACGCAG8400.037.945216
GTACATG601.588099E-435.4304281
GCAGAGT9550.032.9308789
TAATCCT652.5477822E-432.6912544
TTCTAAT550.004437117430.9145972
GTATAAG755.878388E-428.3443431
TGCATAT600.00678898628.338382
GCGGACT300.006802700528.33242222-23
TAAGAGA1054.505766E-628.3324224
Produced by FastQC (version 0.11.5)