FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345370
Sequences flagged as poor quality0
Sequence length61
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7890.22845064713206126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9800.031.731991
GTGGTAT1750.031.4511781
TATCAAC10350.029.4968682
CAACGCA11250.027.618085
ACGGGTA400.00786583927.49985516
GGTATGC400.00786583927.49985531
GTCGGTA400.007871427.49587427
ATCAACG11150.027.38053
TCAACGC11400.027.0174034
AACGCAG11650.026.6698176
CGCAGAG11550.026.6626668
AGAGTAC11700.026.32083711
ACGCAGA11850.026.2196947
TGGTATC2000.026.1286472
CAGAGTA12000.025.89194910
GGTATCA4250.025.2534451
GCAGAGT12700.024.2483319
AGTACGG13250.023.03761513
TATTACC752.864321E-422.0030692
TACGGGG6150.021.46330315
Produced by FastQC (version 0.11.5)