FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161482
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT5590.3461686132200493No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA4300.26628354863080717No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC3500.21674242330414537No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA3170.19630670910689738No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG2830.1752517308430661No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA2710.16782056204406684No Hit
CTATTACGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCACATGCAC2700.16720129797748357No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT2680.16596276984431702No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG2550.15791233697873447No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA2520.15605454477898467No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC2410.14924264004656865No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA2360.1461463197136523No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC2080.12880692584932066No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCT1940.12013722891715485No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT1850.11456385231790539No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCA1820.11270606011815558No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC1810.11208679605157232No Hit
GTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCG1780.1102290038518225No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGT1720.10651341945232286No Hit
GGATATTTGCCGCCTCCCCGAAGCTTATCGCAGGCTATTACGTCTTTCAT1710.10589415538573958No Hit
CCCCGAAGCTTATCGCAGGCTATTACGTCTTTCATCGCCTCTGACTGCCA1700.10527489131915632No Hit
GTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAG1670.1034170991194065No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCT150.001028556884.9873054
GAGTAAG200.003220370763.740481
CGCTTAT302.2510716E-456.6582031
GTATCAA10400.051.8912851
ACACGTT250.0077887950.9923828
TATATTG250.0077887950.9923827
GTTACAG250.0077887950.9923823
TTGGGGA250.0077887950.9923824
TATCAAC12100.044.2495882
AACGCAG12400.043.8644146
TCAACGC12200.043.5385744
CGCAGAG12550.043.3401378
ACGCAGA12600.043.168157
CAACGCA12750.042.6602945
GGAGCGC209.3091873E-442.50023330-31
TCCGATA209.3162875E-442.49365222-23
TGGTATC2100.042.4936522
GTGGTAT2250.041.5493471
ATCAACG13200.040.8840453
GCAGAGT13650.039.8475279
Produced by FastQC (version 0.11.5)