FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161482
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT4540.28114588622880565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3680.22788917650264426No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT2970.18392142777523193No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT2920.18082510744231556No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT2700.16720129797748357No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTG2570.15915086511190102No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGA2210.13685735871490323No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGATA2200.13623809464831993No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA2160.13376103838198683No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG2100.1300454539824872No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTA2060.12756839771615414No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA1980.12261428518348795No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG1810.11208679605157232No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA1720.10651341945232286No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGA1700.10527489131915632No Hit
GTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGA1690.10465562725257305No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCA1660.10279783505282322No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATTTCTTTTC1640.10155930691965669No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATA358.61516E-539.3007661
TAGATAG358.6309126E-539.288592
TGGTATC2050.038.905292
GTGGTAT2150.038.3867951
CGGGGCA401.893518E-434.37751817
TACCGAC401.8969699E-434.366878
TTTGTAC501.8133034E-532.9921954
TCAGGGT350.004087831431.43087237
CCTTAGA350.004094036831.42113714
ACGCGTA350.004094036831.42113744
CATTGTA453.7796193E-430.55779515
CGACCAT453.7864916E-430.5483311
CTTTGTA453.7864916E-430.548333
GTATCAA11000.030.2615911
TTGTACC553.4780947E-529.9929075
TAGCCTC400.007850626527.50201634
CCTTTGT507.0025894E-427.5020122
GTACCGA507.015288E-427.4934967
GACGTTC507.015288E-427.49349626
TATCAAC12900.025.7964632
Produced by FastQC (version 0.11.5)