FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101903
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29760.27007821922619324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6850.032.11612
GTATCAA34400.031.1179081
GTGGTAT8150.028.3622651
TATCAAC40000.027.018172
TCAACGC41150.026.2488044
ATCAACG41300.026.2223993
CAACGCA42450.025.5756055
AGAGTAC40400.025.38700111
AACGCAG42800.025.1107236
CAGAGTA41000.025.07799710
GATCGAC2550.024.80564120
ACGCAGA44050.024.3981597
CGCAGAG44300.024.3247288
CGGGATC4000.024.06416717
GGTATCA16200.023.7811181
GCAGAGT47050.022.6713399
AGTACGG44900.022.47928613
TGCCGAC4950.021.67210855
GAGTACG47300.021.51019112
TACGGGG18250.021.39874515
Produced by FastQC (version 0.11.5)