FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579403
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.36951828002271303No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14340.24749612963688486No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.16758629140684464No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6450.051.388932
GTGGTAT7350.046.2647061
GTATCAA24000.040.911731
TATCAAC27650.035.348212
GGTATCA13600.035.0046921
CGTACGA250.00278775334.0030934-35
ATCAACG29450.032.8991173
TCAACGC29450.032.8991174
CAACGCA29950.032.3498845
AACGCAG30850.031.543876
ACGCAGA32850.029.4914887
CGCAGAG33200.029.0525978
GCAGAGT34850.027.7990079
CAGAGTA23400.025.24039510-11
GTATACT1251.7505003E-523.7970284
GAGTACG25450.022.53944212-13
TAATAGT950.002343414822.365634
GTCTAAG1000.003151343221.252851
AGAGTAC22350.021.19798310-11
GTACGGG26700.020.92722714-15
Produced by FastQC (version 0.11.5)