FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579403
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13560.23403399706249362No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4450.036.4398462
GTGGTAT4850.032.8876841
GTATCAA21200.030.6142181
GTACGCG507.0369715E-427.49378228
TATCAAC23850.026.9657172
GGTATCA9750.026.7957551
TCAACGC25250.025.2485314
ATCAACG25500.025.1065923
TCGGAGC550.001226818424.99650647
TACGCGA550.001226818424.99650629
CCATCGG550.001227436224.99434926
CAACGCA25750.024.8671345
AACGCAG26050.024.5828786
ACGCAGA26550.024.2234427
AGAGTAC24600.024.13879811
CGCAGAG26600.023.8699978
CAGAGTA25150.023.7202210
ACGGGCT1653.6379788E-1223.33611916
TGCCGAC2600.023.28005255
GAATCCG600.002035970822.91346523
Produced by FastQC (version 0.11.5)