FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373637
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5710.1528221241472338No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAA303.4853525E-545.83623525
GTATCAA10700.034.707751
TATCAAC12050.031.491552
TGGTATC2400.029.7895642
GTGGTAT2500.029.7098371
ATCAACG12700.029.4427973
TCAACGC12900.028.7731864
CAACGCA13200.028.119255
AACGCAG13450.027.5965886
ATAACCG400.00786915627.49805643
TCCCGTC400.00787429927.4943757
AGAGTAC13000.027.07501211
ACGCAGA13900.026.7031737
CAGAGTA13400.026.67722110
CGCAGAG14200.026.1390238
GTATAGG651.08459375E-425.3930231
GTAACAC550.001226465724.994893
GCAGAGT14950.024.82779
TACGGGG5950.024.03513315
AGTACGG14100.023.99088113
Produced by FastQC (version 0.11.5)