FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628028
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17120.2725993108587515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5350.035.979171
TGGTATC5250.034.026622
CGGGCTA350.004096224431.43171117
GTATCAA24200.029.7709581
GTTGCGC400.00787273627.49836744
CGCCTAC400.0078788627.49398828
TATCAAC27850.025.953462
TCAACGC28650.025.2267514
GATCGAC1201.0859367E-925.20683520
ATCAACG28900.025.0085243
CAACGCA28950.024.967325
AGAGTAC27650.024.85695611
AACGCAG29550.024.460376
CAGAGTA28400.024.39412910
CGCAGAG30000.023.8224268
ACGCAGA30400.023.6879277
GTATTAT1052.096549E-723.5700281
GGTATCA11500.022.9551561
TAGCACG600.00204276422.900714
GCAGAGT31450.022.8151329
Produced by FastQC (version 0.11.5)