FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338254
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5860.17324259284443053No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGCA300.001923221536.6685416
CGAGCGT401.8981467E-434.37675516
TGGTATC3300.034.163362
GTGGTAT3650.031.6548141
TGCCGAC1101.2732926E-1130.00596855
GCTTATA705.675989E-627.509541
GGGCGTA507.013425E-427.50547237
CGTGCGT507.025564E-427.4973447
GTATCAA12050.026.4822121
AGCGTTA550.001224399825.00127618
TGCGAGC550.001224399825.00127614
TCCGGTC1001.3070894E-724.7439468
CTGCCGA2400.024.06728755
CAAACGA1052.0883817E-723.57263449
TGTCGTT701.7963431E-423.57263232
AACGCAG13700.023.4796566
TCAACGC13950.023.4495754
TATCAAC13850.023.22882
AACGAAC952.3039647E-623.16250251
CACGTAG952.3098983E-623.15565326
Produced by FastQC (version 0.11.5)