FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598927
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT9820.1639598815882403No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT8710.14542673815005835No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA8230.13741240585246617No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA7910.13206951765407138No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.12422215061267902No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC6300.10518811140589755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC5750.053.2137872
GTGGTAT6250.048.9648551
GGTATCA15100.044.75611
GTATCAA29250.042.2862471
TATCAAC33550.036.6070562
TCAACGC34950.035.0161634
ATCAACG35550.034.425173
CAACGCA36350.033.6675345
AACGCAG37050.033.031446
CGCAGAG37350.032.87998
ACGCAGA38400.031.9808397
GCAGAGT40150.030.4810669
CTGGTCG957.4517244E-526.8380459
CAGAGTA34700.025.5328810-11
GAGTACG39400.022.37922912-13
TGTTACG950.002340456222.3706421
GACAGGT2759.094947E-1221.638511
TCTTAAC1000.003156685321.2467863
AGAGTAC34600.020.5713110-11
GTAATAC1455.5062286E-520.514143
Produced by FastQC (version 0.11.5)