FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832471
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.22799593018855915No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12060.14486991138430047No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.10234590754512769No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6500.046.418222
GTATCAA27650.044.589131
GTGGTAT7350.040.488981
TATCAAC32800.037.7018472
ATCAACG33550.036.7323723
TCAACGC33750.036.5146984
CAACGCA33950.036.4247635
AACGCAG34600.035.86336
GGTATCA14600.035.524921
ACGCAGA37550.033.156997
CGCAGAG37650.033.068928
GCAGAGT39850.031.2432849
TATACGG853.492191E-529.9968625
CTAAGGT1351.1161119E-625.1825524
CAGAGTA29150.025.0730610-11
AGAGTAC28650.022.76675610-11
GAGTACG32600.022.09375612-13
GTGTTAG2409.1858965E-1021.2567161
CGGTCGA500.003151567421.25543874-75
AGTACGG31050.020.87015312-13
Produced by FastQC (version 0.11.5)