FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883561
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13910.15743112246919003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6100.031.9956132
GTATCAA27100.030.9608021
GTGGTAT6700.028.3306081
GGTATCA12500.026.1889781
TATCAAC32450.025.7525842
TCAACGC33100.025.1581234
CAACGCA33500.024.9397645
ATCAACG33400.024.9321523
AACGCAG33950.024.4514436
ACGCAGA34400.024.1315827
CGCAGAG34800.023.8555588
AGAGTAC34350.022.7301711
CAGAGTA35300.022.50658610
GCAGAGT37950.021.9478879
TACGGGC5900.021.90932815
ACGGGCT2400.021.77460116
GCTTGTA2600.021.16111
AGTACGG37250.020.67354613
GAGTACG38900.019.93240712
GTTAGGC700.004992999619.630693
Produced by FastQC (version 0.11.5)