FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787427
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16270.20662232816502354No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4500.031.1688272
GTATCAA22300.030.7298851
GTGGTAT4800.030.3879281
GGTATCA9700.028.372311
TATCAAC25100.026.734922
TCAACGC26400.025.7162444
AACGCAG26750.025.2818346
ATCAACG26800.025.231463
CAACGCA27150.025.005855
ACGCAGA28100.024.0672267
CGCAGAG28600.023.5503488
AGAGTAC27100.023.43960811
CAGAGTA28200.022.71887410
GTCTACG852.553779E-522.6644691
TGCCGAC2800.022.59228555
CGGGATT2250.022.00152417
GCAGAGT31100.021.7483929
AGTACGG30300.020.87603413
GAGTACG32800.019.36872112
CTGCCGA5450.019.1768655
Produced by FastQC (version 0.11.5)