FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994667
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25560.2569704232672844No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16920.17010718159946997No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.11521443860105945No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT10760.10817690744741708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9400.050.182552
GTGGTAT10500.046.54661
GGTATCA20300.040.405521
GTATCAA37700.039.342641
TATCAAC39750.037.4186862
TCAACGC42200.035.0448654
ATCAACG42300.034.9620133
CAACGCA42550.034.75665
AACGCAG44200.033.4591266
ACGCAGA47800.030.8487367
CGCAGAG47900.030.7843328
GCAGAGT50750.029.0555559
CAGAGTA34850.026.82598310-11
CGCAACG900.001711622123.6094075
GAGTACG40950.022.62295212-13
TAGTACC1903.8795406E-822.3668084
GTACGGG42000.021.90615714-15
ACGGGGC3500.020.64136716-17
AGAGTAC34250.020.53398110-11
ACCGCTC1254.786744E-420.3975038
Produced by FastQC (version 0.11.5)