FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087308
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17770.1634311529023975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8100.035.306042
GTGGTAT9100.032.6499821
GTATCAA35600.028.592511
CGACGCT400.00787754527.4967127
TATCAAC40950.024.9126972
TCAACGC41600.024.581624
ATCAACG41800.024.3993633
CAACGCA42750.024.1154845
GGTATCA17950.024.0622231
AACGCAG43150.023.8941336
AGAGTAC41700.023.67331911
TGCCGAC3900.023.27394355
ACGCAGA44950.022.998477
CAGAGTA43200.022.84922410
CGCAGAG45300.022.6386978
AGTACGG46450.021.19522313
GCAGAGT48700.021.059149
GAGTACG47700.020.75414312
GCTTGTA3200.020.6329751
GTCCTAG2450.020.2118931
Produced by FastQC (version 0.11.5)