FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964860
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12690.1315216715378397No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7050.031.203882
GTGGTAT7800.029.624431
GGTATCA17050.027.5890711
GTATCAA32850.027.5502721
CGCACCG400.0078886827.4884435
TATCAAC37950.023.911672
TCAACGC38050.023.6234464
AACGCAG38450.023.4503946
CAACGCA38750.023.2676375
AGAGTAC38650.022.97699511
ATCAACG39250.022.9748083
ACGCAGA39900.022.5981867
CGCAGAG40200.022.4295448
CAGAGTA39950.022.22815510
TCGGGTA904.002936E-521.38322425
GCAGAGT44000.020.6195379
GGCCGCT956.0755105E-520.2556937
AGTACGG44150.019.8727413
GCGTAAG700.00498159919.63867228
ACGGGTA1151.20923105E-519.13330316
Produced by FastQC (version 0.11.5)