FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815641
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT11320.13878654947458502No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT8170.10016661742114484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15450.044.5699541
GTATCAA26150.040.7997671
GTGGTAT5600.038.7113041
TGGTATC5700.038.0251662
TATCAAC30450.035.4504852
TCAACGC31650.033.967954
ATCAACG31950.033.6490023
CAACGCA32000.033.463635
AACGCAG33650.032.3278926
CGCAGAG33400.032.315428
ACGCAGA34650.031.3949097
TATACGT550.004450586630.9043646
GCAGAGT37650.028.780469
CAGAGTA34600.025.23819210-11
GGGTACG1051.4685822E-424.2894461
GTATACA1601.8272294E-723.9099241
GTATAAA1601.8272294E-723.9099241
TACACTC1102.0173067E-423.1782725
CTGTTCG1903.880814E-822.3650029
GAGTACG40300.021.40491512-13
Produced by FastQC (version 0.11.5)