FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815641
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28800.35309652163145305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4950.032.2321
GGTATCA13550.028.4219071
GTATCAA26250.028.2942941
TGGTATC5250.028.2769492
GTATAAC1156.6029315E-1026.312331
ACCGTTA550.001228906224.9907178
TATCAAC30150.024.6192342
TCAACGC30650.024.0352754
ATCAACG30900.023.9283073
CAACGCA31650.023.3627175
TGCCGAC4150.023.19553855
AACGCAG32350.023.0285426
CGCAGAG32350.023.0285428
CGATCGT600.002033613422.91940347
CGCTAGG600.002039423422.908167
ACGCAGA32850.022.7617137
AGAGTAC33200.022.61148811
CAGAGTA34000.022.16033410
GCAGAGT34950.021.396689
CTGCCGA7100.020.53061955
Produced by FastQC (version 0.11.5)