FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044596
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22190.21242662234969306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33000.030.1735251
GTGGTAT6350.029.8886831
TATACCG606.3383806E-527.48655
TATCAAC37650.026.6507662
TGGTATC7200.025.5813872
GGTATCA14850.025.3761371
TCAACGC39700.024.9916274
ATCAACG39950.024.9052243
CAACGCA40550.024.6056695
AACGCAG40800.024.45496
CGCAGAG41000.024.2026868
AGAGTAC40600.023.90751811
ACGCAGA41800.023.8709967
CAGAGTA41850.023.45400210
GCAGAGT43900.022.6092879
GTATAGG1254.61132E-822.0050011
AGTACGG45850.020.99412213
GAGTACG46600.020.71227312
TACGGGG18650.019.612915
CGGGGAA3200.018.9078317
Produced by FastQC (version 0.11.5)