FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336887
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34740.2598574150246057No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA358.686252E-539.272065
GTATCAA38100.030.7730311
GTGGTAT7650.028.781551
GGTATCA17150.027.7630791
TATCAAC43150.026.961022
TCAACGC44400.026.3749774
CAACGCA44850.026.0500285
TGGTATC8350.026.0206012
ATCAACG44900.025.8985653
AACGCAG46450.025.2109586
AGAGTAC45350.024.91695811
ACGCAGA47250.024.7250047
CAGAGTA46550.024.62900410
CGCAGAG47700.024.2621448
GCAGAGT50750.022.7507119
AGTACGG50450.022.07524513
GAGTACG52100.021.53124612
TCACGTA1351.0449003E-720.36710225
TAATCGT1651.9572326E-920.0065238
GTACGGG55650.019.71602814
Produced by FastQC (version 0.11.5)