FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756172
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18370.10460251046025104No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49750.029.978851
TGGTATC11600.029.8666972
GTGGTAT12200.028.645251
CGTTAGA400.00787828827.49710826
TATCAAC57200.026.102282
TCAACGC58500.025.4672494
ATCAACG59450.025.108673
CAACGCA59800.024.8690645
AACGCAG61400.024.1328436
AGAGTAC60400.023.71650511
CGCAGAG62850.023.576758
ACGCAGA63350.023.4333957
CAGAGTA61600.023.29846810
GGTATCA24650.022.5498281
GCAGAGT68050.021.7359859
AGTACGG67600.020.95122513
TGCCGAC4800.020.62811955
CCGTTAA804.4424436E-420.62576716
GAGTACG70000.020.35017812
TACGGGG28650.019.48589315
Produced by FastQC (version 0.11.5)