FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597784
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17440.10915117437651146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11050.031.6235121
TGGTATC11100.030.4694862
GTATCAA50050.028.9168011
GGTATCA26100.026.6715721
TATCAAC56650.025.6281382
TCAACGC58550.024.6463324
ATCAACG59300.024.3376623
CAACGCA59800.024.0866935
CGCAGAG60450.023.78528
AGAGTAC59450.023.4960511
AACGCAG61800.023.2627096
CAGAGTA61950.022.90008210
ACGCAGA62650.022.8607737
GCAGAGT65450.022.1795589
AGTACGG64300.021.51267413
GAGTACG66500.020.96507812
GTACGGG72300.018.90407814
TACGGGG27150.018.1315215
TGCCGAC5700.017.85442555
TACGGGA34950.017.78329515
Produced by FastQC (version 0.11.5)