FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858405
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37290.20065593882926486No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22960.12354680492142456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT15250.045.7143251
TGGTATC15500.044.1471182
GTATCAA59050.042.6167261
GGTATCA30700.037.9394041
TCAACGC68750.036.4076274
CAACGCA69600.036.0851065
TATCAAC69600.036.0288932
ATCAACG69650.035.9371763
AACGCAG73800.034.146656
ACGCAGA77050.032.65037
CGCAGAG78850.031.8519138
GCAGAGT84000.029.8484979
CAGAGTA62300.025.34081310-11
AGAGTAC59950.022.3999810-11
GAGTACG69900.022.34240712-13
GTACGGG74400.020.6766214-15
CGGGACG1455.511634E-520.515394
AGTACGG65700.020.37487412-13
CCGTCTT12350.018.41273984-85
ACGGGAT13250.018.28087816-17
Produced by FastQC (version 0.11.5)