FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858405
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG300.001928081436.6583210
GTGGTAT11050.030.618731
GTATCAA53900.029.5994431
TGGTATC10850.029.3918572
GGTATCA25800.028.4666161
ATCAACG61500.025.6532293
TCAACGC61250.025.6226234
CAACGCA62100.025.2719125
TATCAAC63600.024.941212
AACGCAG63600.024.6353136
ACGCAGA65850.023.8770477
AGAGTAC64350.023.58564611
CAGAGTA64750.023.4811410
CGCAGAG67050.023.4497158
GCAGAGT71450.022.0476789
AGTACGG68950.021.73884213
GAGTACG72100.020.82162512
TAACCGG700.00497054819.64737752
TACGGGG28850.019.25660515
GTACGGG76800.019.1229214
Produced by FastQC (version 0.11.5)