FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792314
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39340.21949278976786435No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24210.1350767778413827No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA22570.12592659545146667No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT19490.10874210657284382No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC18920.10556186025439739No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA18000.10042883110883473No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16450.047.8053172
GTGGTAT18350.043.3296281
GTATCAA60900.041.960111
ATCAACG71800.035.6314353
TCAACGC72200.035.316314
GGTATCA33200.035.2187581
TATCAAC72950.035.195082
CAACGCA72750.035.0483365
AACGCAG75450.033.9067656
ACGCAGA81650.031.4344377
CGCAGAG82550.030.9887688
GCAGAGT88950.028.8554639
CAGAGTA62500.025.4289610-11
GAGTACG71300.022.23086412-13
AGAGTAC60050.022.11434410-11
GACAGGT8350.019.8590091
ACGGGTC4650.019.6481916-17
TATACTG4350.019.538445
GTACGGG81100.019.46592314-15
AGTACGG69100.019.18728812-13
Produced by FastQC (version 0.11.5)