FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066040
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.12494840718922366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8200.045.6113132
GTGGTAT9100.043.4498181
GTATCAA27350.040.57231
TATCAAC30050.036.7733042
TCAACGC31100.035.3951034
GGTATCA14600.035.235381
ATCAACG31350.035.1128463
CAACGCA31650.034.780025
AACGCAG34000.032.501116
ACGCAGA35750.031.0232127
CGCAGAG35800.030.7424898
GCAGAGT40500.027.2797649
CAGAGTA28950.023.705910-11
GGACCGT1553.707044E-621.9362346
TTAGACG1000.003154885521.2507294
AGAGTAC27300.020.70243610-11
GAGTACG34100.019.87691712-13
GTACTAT1751.056415E-519.435621
CCGTCTT11200.019.16450184-85
GTACGGG36100.018.65798614-15
Produced by FastQC (version 0.11.5)