FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066040
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTT350.004107811531.415576
GTGGTAT6050.030.9224151
GTATCAA27900.028.8923781
TGGTATC6050.028.6258582
GGTATCA13650.028.0157341
TATCAAC32550.024.4917832
ATCAACG33900.023.4320563
TCAACGC34000.023.2025454
CAACGCA34250.023.1134365
AGAGTAC33700.022.43768511
AACGCAG35750.022.3753576
CAGAGTA34300.022.12535310
ACGCAGA36100.022.0822737
CGCAGAG36800.021.5885478
TGCCGAC5250.020.9555155
GCAGAGT40000.019.865199
AGTACGG37350.019.80698813
GTATAGG1452.203542E-718.9736941
GAGTACG39500.018.93420612
CGGGATA1603.000423E-818.90729917
Produced by FastQC (version 0.11.5)