FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520866
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6750.1295918719977883No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3900.033.1502271
TGGTATC3750.032.986982
CGGGCGA350.004097803531.42824217
GTATCAA16200.030.054641
TGCCGAC2500.028.60244855
TTACACG400.00788071927.491794
TATCAAC18650.026.0889022
TCAACGC18950.025.0980474
ATCAACG19200.024.909653
GGTATCA7650.024.8108121
CAACGCA19200.024.4872265
AACGCAG19450.024.3138436
CGCAGAG19400.024.2371088
AGAGTAC19500.024.11513111
ACGCAGA19850.023.96247
CAGAGTA20600.023.0944210
TACGGGC3300.022.49976315
AGTACGG21200.022.31108913
GTCTACG752.8643513E-422.006111
GCAGAGT21700.021.7949129
Produced by FastQC (version 0.11.5)