FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216842
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22020.1809602232664553No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9300.037.550822
GTGGTAT9850.036.0375821
GTATCAA38600.029.5131261
ACGTTAG400.00788106627.4944528
TATCAAC44350.026.0407982
TGCCGAC4600.025.7087355
TCAACGC45450.025.4658154
CAACGCA46150.025.077495
ATCAACG46350.024.9733873
AACGCAG46800.024.6745076
GGTATCA19650.024.6463321
AGAGTAC45450.024.32152411
CGCAGAG47950.024.025398
ACGCAGA48700.023.711857
CAGAGTA47200.023.59454510
GCAGAGT51850.022.2722179
AGTACGG49750.021.8351513
TACGGGG20950.021.52929515
GAGTACG52300.021.08343112
ACGGGGG6050.020.45630316
Produced by FastQC (version 0.11.5)