FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116356
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.15165413183608095No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.10274500249024504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7650.043.8866462
GTATCAA31500.043.4597431
GTGGTAT8550.040.277341
GGTATCA16850.040.117981
TATCAAC36150.037.971782
TCAACGC36900.037.0831644
ATCAACG37100.036.8832553
CAACGCA37200.036.7841035
AACGCAG38700.035.3583686
ACGCAGA41250.033.1725777
CGCAGAG42250.032.387438
GCAGAGT46100.029.77489
CAGAGTA36150.025.39175810-11
ATATCGC900.001711989823.6088476
GAGTACG41350.021.9416712-13
AGAGTAC35150.021.45953610-11
GTACACT1604.887217E-621.2479634
GTACGGG43650.020.7855214-15
GTGCGCA1708.257739E-619.9980839
CCGTCTT12750.019.83409584-85
Produced by FastQC (version 0.11.5)