FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116356
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11670.10453654568972622No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7450.032.4788552
GTGGTAT7750.031.5962181
CGGGTCG350.004098684531.42998117
GTATCAA31800.028.2922481
GGTATCA15050.026.508081
TATCAAC35700.025.031652
TCAACGC36200.024.8344864
CAACGCA36700.024.4950435
ATCAACG36900.024.3633693
AACGCAG38050.023.6259656
ACGCAGA39100.022.92127
CGCAGAG39400.022.817478
AGAGTAC38400.022.62818111
CAGAGTA40050.021.7626410
GCAGAGT42050.021.2496999
GTATACG650.003244745321.148163
TATACGG650.003245588221.1472155
AGTACGG40800.020.96294813
TGCCGAC4850.020.41420255
GAGTACG43200.019.98663512
Produced by FastQC (version 0.11.5)