FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288308
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30360.23565793273037194No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20190.15671718253709518No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA15540.12062332920388602No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT13330.1034690462218662No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.10245997075233562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC11800.048.6245462
GTGGTAT12850.046.9757081
GTATCAA46400.043.700711
TATCAAC54200.037.404422
TCAACGC54850.037.035774
ATCAACG55700.036.4705923
CAACGCA56000.036.35115
AACGCAG57250.035.5574076
GGTATCA23850.034.0434151
CGCAGAG60600.033.799548
ACGCAGA60450.033.7428177
GCAGAGT64350.031.8298689
CAGAGTA48800.024.99185210-11
AGAGTAC46150.022.88184510-11
GAGTACG55050.022.00006312-13
AGTACGG51700.020.3843812-13
GTATGTA3350.020.3031441
GTACGGG59450.020.26457814-15
ACGGGAT10750.019.567416-17
GCCGTCT15800.019.3688884-85
Produced by FastQC (version 0.11.5)