FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295994
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.13071048168432878No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC11250.042.690462
GTGGTAT12050.039.8670041
GTATCAA37350.039.8381961
GGTATCA20150.034.390211
TCAACGC42850.034.3133544
TATCAAC43400.033.9816632
CAACGCA43750.033.801745
ATCAACG43800.033.5691153
AACGCAG44900.032.9372636
ACGCAGA46800.031.689657
CGCAGAG48800.030.1296568
GCAGAGT54100.027.25659
CAGAGTA40000.024.06279610-11
GTACGAC1302.3736508E-522.8819263
AGAGTAC38700.020.53376210-11
GAGTACG47550.020.19702512-13
CGACCAT1507.15115E-519.8317686
CCGTCTT10550.019.33832484-85
GTACGGT1100.005470008619.3241121
GTACGGG50150.018.89535514-15
Produced by FastQC (version 0.11.5)