FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295994
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8100.031.9179291
TGGTATC8350.030.291832
GTATCAA37950.027.3225611
CCGTTAA1057.2595867E-926.19100816
GGTATCA19200.026.0712591
TATCAAC42250.024.2720412
ATCAACG43100.023.8516223
CAACGCA43450.023.7814255
TCAACGC43150.023.7575344
AACGCAG43850.023.3147056
ACGCAGA44350.023.0518557
CGCAGAG44950.022.6236218
AGAGTAC44100.022.257211
GCCGTTA1254.623689E-822.00044815
TACCGCG1254.6520654E-821.9877138
CAGAGTA45400.021.85790310
GGCCGTT1307.003473E-821.1534614
GCAGAGT49800.020.4778779
AGTACGG48750.020.25091213
ACGGCCG2053.6379788E-1220.12935438
Produced by FastQC (version 0.11.5)