FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245036
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41440.3328417812818264No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6400.029.2318381
GTATCAA31450.029.0432111
ACGTACG507.025408E-427.5045834
TGGTATC6700.026.6814042
GGTATCA16900.026.0471651
TATCAAC36550.025.1321262
ATCAACG37450.024.44983
TCAACGC37500.024.3419194
CAACGCA38500.023.7096615
AACGCAG40550.022.782246
ACGCAGA40550.022.7144367
AGAGTAC39950.022.3003211
CGCAGAG41250.022.2623238
CAGAGTA42050.021.44647210
GTATAAC1055.497066E-620.9617651
AGTACGG42850.020.53770313
GCAGAGT44850.020.4753849
GAGTACG45150.019.67184612
TGCCGAC3250.019.4647855
GTGTATA2201.0913936E-1118.7583981
Produced by FastQC (version 0.11.5)