FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511994
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28700.18981556805119595No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT27180.1797626181056274No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22000.14550322289638715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46000.042.2328531
TGGTATC12600.042.155972
GTGGTAT13050.040.718481
GGTATCA25900.036.9296151
TATCAAC53100.036.65142
TCAACGC55700.035.0168574
ATCAACG55850.034.6945573
CAACGCA56000.034.525745
AACGCAG59000.032.9142386
CGCAGAG63500.030.6486538
ACGCAGA64800.029.9682147
GCAGAGT70150.027.6826829
CAGAGTA50250.024.2697610-11
GAGTACG56350.021.45469512-13
AGAGTAC47500.021.02296310-11
GACAGGT7350.020.8212681
GTAATTA6200.020.5693991
GTACGGG63050.019.07403214-15
TATGTCG1358.0418657E-418.8858765
AGTACGG54700.018.10100212-13
Produced by FastQC (version 0.11.5)