FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185580
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT30160.25439025624588807No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15180.12803859714232696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8300.047.6272662
GTATCAA38800.043.8354641
GTGGTAT9300.042.5204051
GGTATCA19200.040.30581
TATCAAC43900.038.5362432
TCAACGC44800.037.5675624
CAACGCA45650.036.8680575
ATCAACG46050.036.6401023
AACGCAG47400.035.5965586
ACGCAGA50750.033.244037
CGCAGAG50950.033.0301258
GCAGAGT55000.030.752449
CAGAGTA42250.026.15205610-11
GAGTACG49850.022.07973112-13
AGAGTAC41400.022.06974610-11
GTACGGG52700.020.48246814-15
AGTACGG46300.019.7340712-13
GCTTATA3105.4569682E-1119.2027651
ACGGGAT10450.017.4868416-17
TACGGGG19700.017.47342314-15
Produced by FastQC (version 0.11.5)