FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185580
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63300.5339158892693872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7300.033.908352
GGTATCA18950.028.8907741
GTGGTAT8400.028.4941221
GTATCAA37150.028.066981
TATCAAC42550.024.3038642
TCAACGC43450.023.724134
CAACGCA44250.023.480595
AACGCAG44450.023.3759256
ATCAACG44550.023.2029883
ACGCAGA45600.022.7864047
CGCAGAG46400.022.4537248
AGAGTAC45800.022.21424711
CAGAGTA47000.021.8216710
TGCCGAC5450.021.19620755
GCAGAGT50250.020.7377629
TACGCGA1501.43281795E-820.16493429
AGTACGG49950.019.9307413
GAGTACG51500.019.59540412
AGACGGT1009.0176545E-519.2418526
TACGGGT5250.018.3340815
Produced by FastQC (version 0.11.5)