FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915377
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58750.6418120621339624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6100.030.2135371
TGGTATC6150.029.4980342
GTATCAA27800.028.5962851
GGTATCA13500.027.7116091
ACGAGAC400.00789707927.48233
TATCAAC31850.025.2861332
CAACGCA32700.024.4553895
TCAACGC32500.024.4381054
AACGCAG33050.024.2835356
ATCAACG32700.024.2045963
ACGCAGA34050.023.5716517
CGCAGAG33900.023.4327038
AGAGTAC32850.023.35006111
CAGAGTA33850.022.74022710
GCAGAGT36050.022.0388039
AGTACGG35200.021.64206713
ACGGGAT6650.021.50519216
TCGACAC2200.021.24792922
GAGTACG36750.020.95148712
TGCCGAC4550.020.55868755
Produced by FastQC (version 0.11.5)