FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626649
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.1166522247701664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19150.043.2863961
GTGGTAT4150.043.0216181
TGGTATC4300.040.522572
GGTATCA9600.038.9670031
TCAACGC21850.038.1229254
TATCAAC22150.037.798462
ATCAACG22050.037.7771383
CAACGCA22700.036.8826375
AACGCAG24200.034.9477586
ACGCAGA25700.032.7426387
CGCAGAG26000.032.3648388
TTAACGA550.004446848330.9085665
GCAGAGT27800.030.2692749
CAGAGTA22150.024.271610-11
CCTAGGC2002.6029738E-923.3746053
AGAGTAC21250.023.0996110-11
GTACTAT1302.3648956E-522.8897081
TAGGCTC1553.7046502E-621.9351145
GTATATA1754.3298496E-721.8620071
GAGTACG24900.021.50566112-13
Produced by FastQC (version 0.11.5)