FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876739
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17580.20051577493415945No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11830.1349318326206545No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8050.052.2692222
GTGGTAT8700.048.377851
GTATCAA33950.044.580081
TATCAAC37050.040.723662
TAGGTCG559.5673124E-538.6313365
TCAACGC39250.038.3262184
ATCAACG39900.037.808363
CAACGCA40150.037.4671065
AACGCAG41100.036.7044686
ACGCAGA43800.034.6339267
CGCAGAG43600.034.6018268
GGTATCA16150.032.9054681
GCAGAGT47150.031.9966019
CAGAGTA34900.025.38854410-11
GTATTAC2251.4551915E-1124.5635661
AGAGTAC33250.023.90050710-11
GTCTAGT900.00170749723.6188161
GAGTACG38400.022.85314612-13
ACGGGAT6550.022.38130616-17
GTACGGG40650.021.43017414-15
Produced by FastQC (version 0.11.5)