FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876739
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11870.13538806874109627No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG350.004098836331.42899546
GTGGTAT6800.031.1472381
GTATCAA31950.031.0795021
TGGTATC6400.030.4995212
TATCAAC34550.028.646342
TCAACGC35200.027.9531754
ATCAACG35300.027.9518473
CAACGCA35600.027.6438245
GATCGTA400.00787161327.5003748
CGATCGT400.00787161327.5003747
AGAGTAC35500.027.2648611
AACGCAG36500.027.1174626
GGTATCA14150.027.0206681
CGCAGAG37000.026.7510138
CAGAGTA37000.026.6054310
ACGCAGA37400.026.4649057
ACGGGAG4550.025.38785616
GCAGAGT39650.025.1740119
AGTACGG39750.024.28473313
GAGTACG40150.024.10853812
Produced by FastQC (version 0.11.5)