FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714125
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11880.16635743042184492No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4600.029.3192021
TGGTATC4650.028.9795652
GTATCAA22650.028.4355471
GGTATCA10900.028.2816961
TATCAAC25800.024.836192
TACGGGC4300.024.30152115
TCAACGC26200.024.1336614
ATCAACG26550.023.818853
CAACGCA26750.023.7402275
AGAGTAC26750.023.33404211
CAGAGTA27350.023.22429510
AACGCAG27650.022.8680616
ACGCAGA27900.022.663157
CGCAGAG28300.022.537118
GTCGCTC752.8731854E-421.99773228
ACGGGCA2800.021.60642816
AGTACGG29250.021.2471613
AGTTCGC650.00324445221.147228
GCAGAGT30200.021.122179
GAGTACG29750.020.98103112
Produced by FastQC (version 0.11.5)