FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838982
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11910.14195775356324689No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22450.042.217871
TGGTATC6100.039.0157622
GGTATCA11800.038.1796231
GTGGTAT6400.037.8532071
TATCAAC26850.035.613952
TCAACGC27000.035.256594
ATCAACG27400.034.896993
CAACGCA27550.034.552745
AACGCAG28550.033.3424846
ACGCAGA30150.032.136877
CGCAGAG31250.030.7336738
GCAGAGT33950.028.4146399
TACCGAA755.906927E-428.3311867
CAGAGTA25350.023.88872310-11
GTAATAC1703.2833668E-722.5009781
TATACGG950.00234361422.3667285
CGGTTAA1353.174239E-522.035379
GAGTTAT1754.3409455E-721.8580931
GTCGTAC1000.00315416321.2509251
AGACCGT1000.003156344621.2483926
Produced by FastQC (version 0.11.5)